Cytotoxic Effects of Aflatoxin B1 Standard in Relation to Aflatoxin Extracts from South African Compound Feeds on Human Lymphocytes
نویسنده
چکیده
Cytotoxicity testing of aflatoxin (AF) on the viability of cells grown in cultures can be widely used to predict the potential toxic effects of AF in animals. To this end, an in vitroexperimental study was conducted to ascertain the toxic effects of AF extracts obtained from compound feeds in South Africa on human lymphocytes in comparison to that of an AFB1 standard. The approach adopted was on the basis of viable cells reducing methyl tetrazolium bromide (MTT) from blue to a purple formazan dye, which was then spectrophotometrically quantified to provide the rate of cytotoxicity.
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